Morphological and Molecular Characterization of Fall Armyworm, Spodoptera Frugiperda, From Selected Regions in Kenya.
DOI:
https://doi.org/10.47941/ijbs.1808Keywords:
Spodoptera Frugiperda, Strain, Corn, RiceAbstract
Purpose: This study was done in Kenya for the purpose of confirmation and tentative identification of fall armyworm to help in proper monitoring and effective management of the pest. To achieve this, the study was organized in order to characterize Spodoptera frugiperda (fall army worm) found in Kenya using morphological and molecular techniques and determine prevalent strain of Spodoptera frugiperda in eastern and central regions of Kenya. The study was also organized to compare the host diversity for Spodoptera frugiperda strains in eastern and central regions of Kenya.
Methodology: The study was a cross-sectional study which was conducted in 5 regions namely; Machakos, Nyeri, Murang’a, Embu and Kiambu. Sampling of sub counties was done followed by random choice of at least 2 villages and eventually reaching the actual households. Questionnaires were used to interrogate farmers about their knowledge on fall armyworm and seeking permission to check and pick the fall armyworm from their farms. Samples of moths and larvae were obtained. The pest was identified morphologically in the field before being taken to the lab for DNA was extraction and COI gene amplification. The amplified DNA was shipped to Macrogen, Netherlands for sequencing.
Findings: This study confirmed actual establishment of Spodoptera frugiperda in eastern and central Kenya using COI gene amplification and analysis. Phylogenetic analysis indicated the presence of both the “Rice” and “Corn” strains. Results indicated higher prevalence of the Rice strain at 77.8% while that of Corn strain was 22.2%. Investigation of the host plants for the fall armyworm gave no evidence of plant host specificity for R- strain since it was also found in Zea mays (maize). Only two plants species, maize and sorghum, were found to host Spodoptera frugiperda in the study region, with a higher preference towards the maize crop.
Unique Contribution to Theory, Policy and Practice: The findings of morphological and molecular characterization together with phylogenetic studies confirms presence of rice and corn strains of Spodoptera frugiperda in Kenya. The pest was recorded mainly in maize and sorghum crop and due to its host range in its native Western Hemisphere and migration ability it may spread to other crops like millet, rice, cotton, vegetables etc. Host status should be continuously investigated. It was also found that Spodoptera frugiperda has established itself in central and eastern regions of Kenya meaning that permanent solution to control its effects have to be developed.
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References
United States Department of Agriculture. (2018). www.usda.gov › media › blog2. Nagoshi, R. N., Goergen, G., Plessis, H. D., van den Berg, J., & Meagher Jr, R. (2019). Genetic comparisons of fall armyworm populations from 11 countries spanning sub-Saharan Africa provide insights into strain composition and migratory behaviors. Scientific reports, 9(1), 8311.3. World Vision International. (2017). www.wvi.org › gallery › fall-armyworm-invasion-kenya.
De Groote, H., Kimenju, S. C., Munyua, B., Palmas, S., Kassie, M., & Bruce, A. (2020). Spread and impact of fall armyworm (Spodoptera frugiperda JE Smith) in maize production areas of Kenya. Agriculture, ecosystems & environment, 292, 106804.
Gichere, S. N., Khakame, K. S., & Patrick, O. (2022). Susceptibility evaluation of fall armyworm (Spodoptera frugiperda) infesting maize in Kenya against a range of insecticides. Journal of Toxicology, 2022.7.
Burtet, L. M., Bernardi, O., Melo, A. A., Pes, M. P., Strahl, T. T., & Guedes, J. V. (2017).Managing fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), with Bt maize and insecticides in southern Brazil. Pest management science, 73(12), 2569-2577.
Murúa, M. G., Nagoshi, R. N., Dos Santos, D. A., Hay-Roe, M. M., Meagher, R. L., & Vilardi, J. C. (2015). Demonstration using field collections that Argentina fall armyworm populations exhibit strain-specific host plant preferences. Journal of economic entomology, 108(5), 2305-2315.
Katrina, A. S., Kurt, L., Caroline, P. D. B., Rahul, B., Robert, M., Eliseu, P., ... & Juan Luis, J. F. (2021). Whole genome comparisons reveal panmixia among fall armyworm (Spodoptera frugiperda) from diverse locations.
Nagoshi, R. N., Goergen, G., Tounou, K. A., Agboka, K., Koffi, D., & Meagher, R. L. (2018). Analysis of strain distribution, migratory potential, and invasion history of fall armyworm populations in northern Sub-Saharan Africa. Scientific reports, 8(1), 1-10.
Sarkowi, F. N., & Mokhtar, A. S. (2021). The fall armyworm (Faw) spodoptera frugiperda: A review on biology, life history, invasion, dispersion and control. Outlooks on Pest Management, 32(1), 27-32.
Nagoshi, R. N., Goergen, G., Koffi, D., Agboka, K., Adjevi, A. K. M., Du Plessis, H., ... & Brévault, T. (2022). Genetic studies of fall armyworm indicate a new introduction into Africa and identify limits to its migratory behavior. Scientific Reports, 12(1), 1941.
Liu, H., Lan, T., Fang, D., Gui, F., Wang, H., Guo, W and Sahu, S. K. (2019). Chromosome level draft genomes of the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), an alien invasive pest in China. bioRxiv, 671560.
Assefa, F., & Ayalew, D. (2019). Status and control measures of fall armyworm (Spodoptera frugiperda) infestations in maize fields in Ethiopia: A review. Cogent Food & Agriculture, 5(1), 1641902.
Nagoshi, R. N., Goergen, G., Tounou, K. A., Agboka, K., Koffi, D., & Meagher, R. L. (2018). Analysis of strain distribution, migratory potential, and invasion history of fall armyworm populations in northern Sub-Saharan Africa. Scientific reports, 8(1), 1-10.
Navasero, M. V., Navasero, M. M., Burgonio, G. A. S., Ardez, K. P., Ebuenga, M. D., Beltran, M. J. B., ... & Aquino, M. F. G. M. (2019). Detection of the fall armyworm, Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae) using larval morphological characters, and observations on its current local distribution in the Philippines. Philipp. Entomol, 33(2), 171-184.
Michael, O.H., Tay WT., Walsh T.K., Kanyesigye, D., Adumo, S., Abongosi, J, et al. (2018).Detection of sister-species in invasive populations of the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) from Uganda. PLoS ONE 13(4): e0194571. https://doi.org/10.1371/journal.pone.0194571.
Acharya, S., Kaphle, S., Upadhayay, J., Pokhrel, A., & Paudel, S. (2020). Damaging Nature of Fall Armyworm and Its Management Practices in Maize: A Review. Tropical Agrobiodiversity, 1, 82-85.
Bhavani, B., Chandra Sekhar, V., Kishore Varma, P., Bharatha Lakshmi, M., Jamuna, P., & Swapna, B. (2019). Morphological and molecular identification of an invasive insect pest, fall army worm, Spodoptera frugiperda occurring on sugarcane in Andhra Pradesh, India. Journal of Entomology and Zoology Studies, 7(4), 12-18.
Prasanna, B. M., Huesing, J. E., Eddy, R., & Peschke, V. M. (2018). Fall armyworm in Africa: a guide for integrated pest management.
Harrison, R. D., Thierfelder, C., Baudron, F., Chinwada, P., Midega, C., Schaffner, U., and van den Berg, J. (2019). Agro-ecological options for fall armyworm (Spodoptera frugiperda JE Smith) management: providing low-cost, smallholder friendly solutions to an invasive pest. Journal of environmental management, 243, 318-330.
Goergen G, Kumar PL, Sankung SB, Togola A, Tamò M (2016) First Report of Outbreaks of the Fall Armyworm Spodoptera frugiperda (J E Smith) (Lepidoptera, Noctuidae), a New Alien Invasive Pest in West and Central Africa. PLoS ONE 11(10): e0165632. https://doi.org/10.1371/journal.pone.0165632
Babu, S. R., Kalyan, R. K., Joshi, S., Balai, C., Mahla, M., & Rokadia, P. (2019). Report of an exotic invasive pest the fall armyworm, Spodoptera frugiperda (JE Smith) on maize in Southern Rajasthan. Journal of Entomology and Zoology Studies, 7(3), 1296-1300.
Nagoshi, R. N., Dhanani, I., Asokan, R., Mahadevaswamy, H. M., Kalleshwaraswamy, C. M., Sharanabasappa, & Meagher, R. L. (2019). Genetic characterization of fall armyworm infesting South Africa and India indicate recent introduction from a common source population. PLoS One, 14(5), e0217755.
Gichuhi, J., Sevgan, S., Khamis, F., Van den Berg, J., du Plessis, H., Ekesi, S., & Herren, J. K. (2020). Diversity of fall armyworm, Spodoptera frugiperda and their gut bacterial community in Kenya. PeerJ, 8, e8701.
Nayyar, N., Gracy, R. G., Ashika, T. R., Mohan, G., Swathi, R. S., Mohan, M., ... & Venkatesan, T. (2021). Population structure and genetic diversity of invasive Fall Armyworm after 2 years of introduction in India. Scientific reports, 11(1), 1-12.
Wang, X. Y., Wang, M. M., Chen, C., & Wang, X. Q. (2020). Genetic variation and phylogeographic structure of Spodoptera exigua in western China based on mitochondrial DNA and microsatellite markers. PloS one, 15(5), e0233133.
Baudron, F., Zaman-Allah, M. A., Chaipa, I., Chari, N., & Chinwada, P. (2019). Understanding the factors influencing fall armyworm (Spodoptera frugiperda JE Smith) damage in African smallholder maize fields and quantifying its impact on yield. A case study in Eastern Zimbabwe. Crop protection, 120, 141-150.
Lestari, P., Budiarti, A., Fitriana, Y., Susilo, F. X., Swibawa, I. G., Sudarsono, H., ... & Hartaman, M. (2020). Identification and genetic diversity of Spodoptera frugiperda in Lampung Province, Indonesia.
Navasero, M. V., Navasero, M. M., Burgonio, G. A. S., Ardez, K. P., Ebuenga, M. D., Beltran, M. J. B., ... & Aquino, M. F. G. M. (2019). Detection of the fall armyworm, Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae) using larval morphological characters, and observations on its current local distribution in the Philippines. Philipp. Entomol, 33(2), 171-184.29.
Nagoshi, R. N. (2009). Can the amount of corn acreage predict fall armyworm (Lepidoptera: Noctuidae) infestation levels in nearby cotton?. Journal of Economic Entomology, 102(1), 210-218.
Omuut, G., Mollel, H. G., Kanyesigye, D., Akohoue, F., Adumo Aropet, S., Wagaba, H., & Otim, M. H. (2023). Genetic analyses and detection of point mutations in the acetylcholinesterase-1 gene associated with organophosphate insecticide resistance in fall armyworm (Spodoptera frugiperda) populations from Uganda. BMC genomics, 24(1), 1-15.
University of Minesouri, Published by Division of Plant Sciences, 52 Agriculture Lab, Columbia, MO 65211 | plantscience@missouri.edu 573-882-3001. 2020
Mastrangelo, T., Paulo, D. F., Bergamo, L. W., Morais, E. G. F., Silva, M., Bezerra-Silva, G., & Azeredo-Espin, A. M. L. (2014). Detection and genetic diversity of a heliothine invader (Lepidoptera: Noctuidae) from north and northeast of Brazil. Journal of economic entomology, 107(3), 970-980.
Nagoshi, R. N. (2019). Evidence that a major subpopulation of fall armyworm found in the Western Hemisphere is rare or absent in Africa, which may limit the range of crops at risk of infestation. PloS one, 14(4), e0208966.
Wright, S. (1965). The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution, 395-420.
Luo, Z., Brock, J., Dyer, J. M., Kutchan, T., Schachtman, D., Augustin, M., ... & Abdel-Haleem, H. (2019). Genetic diversity and population structure of a Camelina sativa spring panel. Frontiers in plant science, 10, 184.
Nagoshi, R. N., Nagoshi, B. Y., Cañarte, E., Navarrete, B., Solórzano, R., & Garcés-Carrera, S. (2019). Genetic characterization of fall armyworm (Spodoptera frugiperda) in Ecuador and comparisons with regional populations identify likely migratory relationships. PLoS One, 14(9), e0222332.
Lewter, J. A., Szalanski, A. L., Nagoshi, R. N., Meagher Jr, R. L., Owens, C. B., & Luttrell, R. G. (2006). Genetic variation within and between strains of the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae). Florida Entomologist, 89(1), 63-68.
Eweleit, L., Reinhold, K., & Sauer, J. (2015). Speciation progress: a case study on the bushcricket Poecilimon veluchianus. PloS one, 10(10), e0139494.
Herlinda, S., Simbolon, I. M. P., & Suwandi, S. (2022, April). Host Plant Species Of The New Invasive Pest, Fall Armyworm (Spodoptera Frugiperda) In South Sumatra. In IOP Conference Series: Earth and Environmental Science (Vol. 995, No. 1, p. 012034). IOP Publishing.
Casmuz, A., Juarez, M. L., Socias, M. G., Murua, M. G., Prieto, S., Medina, S., ... & Gastaminza, G. (2010). Review of the host plants of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae). Rev. Soc. Entomol. Argent, 69(3-4), 209-231.
Herlinda, S., Simbolon, I. M. P., & Suwandi, S. (2022, April). Host Plant Species Of The New Invasive Pest, Fall Armyworm (Spodoptera Frugiperda) In South Sumatra. In IOP Conference Series: Earth and Environmental Science (Vol. 995, No. 1, p. 012034). IOP Publishing.
Montezano, D. G., Sosa-Gómez, D. R., Specht, A., Roque-Specht, V. F., Sousa-Silva, J. C., Paula-Moraes, S. D., ... & Hunt, T. E. (2018). Host plants of Spodoptera frugiperda (Lepidoptera: Noctuidae) in the Americas. African entomology, 26(2), 286-300.
Fiteni, E., Durand, K., Gimenez, S., Meagher, R. L., Legeai, F., Kergoat, G. J., ... & Nam, K. (2022). Host-plant adaptation as a driver of incipient speciation in the fall armyworm (Spodoptera frugiperda). BMC Ecology and Evolution, 22(1), 1-11.
Ali, M. P., Haque, S. S., Hossain, M. M., Bari, M. N., Kabir, M. M. M., Roy, T. K., ... & Krupnik, T. J. (2023). Development and demographic parameters of Fall Armyworm (Spodoptera frugiperda JE Smith) when feeding on rice (Oryza sativa). CABI Agriculture and Bioscience, 4(1), 29.
Paredes-Sánchez, F. A., Rivera, G., Bocanegra-García, V., Martínez-Padrón, H. Y., Berrones-Morales, M., Niño-García, N., & Herrera-Mayorga, V. (2021). Advances in control strategies against Spodoptera frugiperda. A review. Molecules, 26(18), 5587.42.55.
Rojas, J. C., Kolomiets, M. V., & Bernal, J. S. (2018). Nonsensical choices? Fall armyworm moths choose seemingly best or worst hosts for their larvae, but neonate larvae make their own choices. PloS one, 13(5), e0197628
Padhee, A. K., & Prasanna, B. M. (2019). The emerging threat of Fall Armyworm in India. Indian Farming, 69(1), 51-54.
Juárez, M. L., Schöfl, G., Vera, M. T., Vilardi, J. C., Murúa, M. G., Willink, E., ... &
Groot, A. T. (2014). Population structure of S podoptera frugiperda maize and rice host
forms in S outh A merica: are they host strains? Entomologia Experimentalis et
Applicata, 152(3), 182-199.
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