Bacteriological and Biochemical Study of Natural Honey Available on the Mbujimayi Market

Authors

  • Crispin Muamba Mukendi Official University of Mbujimayi, DRC, General hospital Bonzola, DRC
  • Claris Mpingiyabu Musangu Official University of Mbujimayi, DRC
  • Trésor Kabuya Kabamba Official University of Mbujimayi, DRC University of Heinrich-Heine, Germany
  • Roger Kamwema Shamuana Official University of Mbujimayi, DRC Paris-Cité University, France
  • Séraphin Binene Katulondi Official University of Mbujimayi, DRC
  • Alidor Mbangila Yombo Official University of Mbujimayi, DRC
  • Anaclet Tshinyangu Kandanda Official University of Mbujimayi, DRC
  • André Mutombo Kabamba Official University of Mbujimayi, DRC
  • Joseph Ngandu Tshilunda Official University of Mbujimayi, DRC
  • Gabriel Wakunga Warach University of Lubumbashi, DRC
  • Séverin Uwonda Akinja Official University of Mbujimayi, DRC

DOI:

https://doi.org/10.47941/ijhmnp.3170

Keywords:

Bacteriological and Biochemical Study, Natural Honey, Mbujimayi

Abstract

Purpose: to study the sterility and biochemical composition of natural honey available on the market in the town of Mbujimayi.

Methods: This is a descriptive cross-sectional study, carried out from January 1st to April 30th 2023, a period of 4 months. The study was carried out in two laboratories in the Democratic Republic of Congo: the bacteriological laboratory of the Clinique MIBA located in the commune of Kanshi, city of Mbujimayi, province of Kasaï Oriental for the bacteriological study, and the Centre de Recherche Agro-alimentaire (CRAA) located in the commune of Lubumbashi, city of Lubumbashi, province of Haut-Katanga for the biochemical study. The study sample consisted of natural honey from three local origins: Kabinda (Lomami), Sankuru (Lusambo) and Kanyama (Haut Lomami), chosen on the basis of the availability of their honeys on the market in the town of Mbujimayi. The biochemical study consisted in measuring: water content, PH, sugars, proteins, protein identification, and mineral elements by atomic emission spectrophotometry. Germ testing included yeast, staphylococcus aureus, enterobacteria and salmonella.

Results: Bacteriologically, all three samples were sterile. Biochemically, the three honey samples contained four biochemical substances: carbohydrates, proteins, vitamins (B and C) and minerals (copper, iron, manganese, calcium, magnesium, zinc, sodium, potassium, nickel).

Unique contribution to theory, practice and policy: Local honey from Mbujimayi is sterile whatever its origin (all three origins). The biochemical composition was almost identical, with good antioxidant, antibacterial, nutritional and healing properties. 

Downloads

Download data is not yet available.

Author Biographies

Crispin Muamba Mukendi, Official University of Mbujimayi, DRC, General hospital Bonzola, DRC

Department of Surgery 

Department of Surgery

Claris Mpingiyabu Musangu, Official University of Mbujimayi, DRC

Faculty of Public Health

Trésor Kabuya Kabamba, Official University of Mbujimayi, DRC University of Heinrich-Heine, Germany

Department of Surgery

Vascular and Endovascular Surgery Clinic

Roger Kamwema Shamuana, Official University of Mbujimayi, DRC Paris-Cité University, France

Department of Internal Medicine

 

Endocrinology-Diabetology-Nutrition Unit

Séraphin Binene Katulondi, Official University of Mbujimayi, DRC

Department of Surgery

Alidor Mbangila Yombo, Official University of Mbujimayi, DRC

Department of Surgery

Anaclet Tshinyangu Kandanda, Official University of Mbujimayi, DRC

Faculty of Agricultural Sciences

André Mutombo Kabamba, Official University of Mbujimayi, DRC

Department of Pediatrics

Joseph Ngandu Tshilunda, Official University of Mbujimayi, DRC

Department of Surgery

Gabriel Wakunga Warach, University of Lubumbashi, DRC

Department of Surgery

Séverin Uwonda Akinja, Official University of Mbujimayi, DRC

Department of Surgery

References

Baudel M. Apitherapy. Pharmaceutical Sciences. University of Picardy, 2017.

Bendrer N, Messaouda A. Antibacterial effect of some varieties of Algerian honey. University Ibn Khaldoun-Tiaret, 2021.

Şireli S, Saylak M. The effect of storage temperature on some physicochemical properties of flower honey. J Ethn Foods. 2024;11(1):10. doi:10.1186/s42779-024-00229-3

Ghramh H, Ali Khan K, Alshehri A. Antibacterial potential of honeys from the Asian region against selected pathogenic bacteria. Saudi Journal of Biological Sciences, 2018; 26(6):1278–84.

Abdellah F, Makhloufi C, Boukraa L, Hammoudi Si M, Safa A, Delle N et al. Physicochemical properties, antibacterial and antioxidant activities of two honey varieties from the Algerian Steppe. Journal of Apitherapy and Nature, 2020; 3(2):59–74.

Achouri I, Aboussaleh Y, Sbaibi R, Chemissi H, Bengueddour R. Comparison of physicochemical quality of Ziziphus sp. (Sidr) and Acacia sp. (Samar) honeys consumed in the United Arab Emirates. Innovative Space of Scientific Research Journals, 2015; 10(1):184–91.

Almeida AMM, Oliveira MBS, Costa JG, Valentim IB, Goulart MOF. Antioxidant capacity, physicochemical and floral characterization of honeys from Northeast Brazil. Revista Virtual de Química, 2016.

Amessis N, Amiot MJ, Mansour K. Physicochemical properties and biological activities of some honeys. Food Sciences. University M’hamed Bouguera, 2015; 113 pages.

Bakchiche B, Habati M, Benmebarek A, Gherib A. Physicochemical characteristics, phenolic compound concentrations and antioxidant capacity of four local honey varieties (Algeria). Rev. Mar. Sci. Agron., 2017; 6(1):118–23.

Belhaj O, Oumato J, Zrira S. Physicochemical study of some Moroccan honeys. Rev. Mar. Sci. Agron., 2015; 3(3):71–75.

Benabdallah A. Ecophysiological study, development and importance of medicinal plants of the Mentha genus in the El-Kala National Park (Northeast Algeria). University of Constantine 1, 2017.

Benbareka O, Hafsaoui I. Study of the antibacterial activity of honey harvested in Algeria. University Saad Dahleb-Blida, 2019; 82 pages.

Benkhoucha K, Chenawi K, Mazouni R. Physicochemical and pollen analysis of some Algerian honeys. University Djilali Bounaama of Khemis-Miliana, 2020; 112 pages.

Benzohra A, Ben Saada H. Physicochemical and pollen analysis of honeys produced in different regions. University Djilali Bounaama-Khemis Miliana, 2017.

Boudjelloua R. Contribution to the study of honey consumption in the Tizi-Ouzou region. University Mouloud Mammeri, 2018.

Haskoury R, Kriaa W, Lyoussi B, Makni M. Honeys from Morocco: Physicochemical properties, mineral contents, and antioxidant activities. Journal of Food and Drug Analysis, 2018; 2(6):67–73.

Oskouei ET, Najafi M. Traditional and modern uses of natural honey in human diseases: a review. J. Basic Med. Sci., 2018; 16(7):31–42.

Echchoui A, Benyachou M, El Aissaoui I, Al Ahmad A, Hafidi J, El Mazaiz S, et al. The relevance of honey as an alternative in the treatment of chronic wounds. Ann. Afr. Med., 2014; 7(3).

Kone SGN, Touré S, Bana A, Kone S, Dogba E. Honey in skin wound treatment. Mali Méd., 2016; 31(3).

Mukherjee D, Azamthulla M, Santhosh S, Dath G, Ghosh A, Natholia R et al. Development and characterization of chitosan-based hydrogels as wound dressing materials. J. Drug Deliv. Sci. Technol., 2018; 46:498–510.

Haskoury R, et al. [duplicate of reference 16].

Imtara H, Elamine Y, Lyoussi B. Physicochemical characterization and antioxidant activity of Palestinian honey samples. Food Science and Nutrition, 2018; 6(8):2056–65.

Korichi N, Latamene. Physicochemical and palynological analysis and antibacterial effect of some honeys from Bejaïa. University Abderrahmane Mira of Bejaia, 2017.

Krichen S, Guetatlia I. Evaluation of the antibacterial activity of seven honey samples from Guelma and Tipaza regions. University of Guelma, 2019.

Hallouz MFZ, Mamoun NO. Physicochemical study and evaluation of biological activities of jujube honeys. University Ibn Khaldoun-Tiaret, 2020.

Haderbache L, Annou S, Mohammedi A. Antimicrobial potential of Ziziphus and Euphorbia honeys harvested in a semi-arid region of Algeria and their possible use in soft medicine. Journal of Microbiology, 2020.

Yahiaoui, Soualmia S, Hamadouch S, Nadira. Effect of Sidr honey on the reproductive system and some biochemical parameters, and its impact on wound healing in Wistar albino rats. University Oum El Bouaghi, 2018.

Olivier P. Getting Started in Beekeeping. Afnil-France, 2017; 121 pages.

Laredj H, Rezzoug W. Microbiological and physicochemical characterization of honeys from the Tiaret region of Algeria. Asian Journal of Pharmaceutical Research and Health Care, 2017.

Nascimento K, Sattler JAG, Macedo LFL, González CVS, Pereira MIL, Araújo SE. Phenolic compounds, antioxidant capacity and physicochemical properties of Brazilian Apis mellifera honeys. LWT – Food Science and Technology, 2018; 91:85–94.

Downloads

Published

2025-09-15

How to Cite

Mukendi, C. M., Musangu, C. M., Kabamba, T. K., Shamuana, R. K., Katulondi, S. B., Yombo, A. M., … Akinja, S. U. (2025). Bacteriological and Biochemical Study of Natural Honey Available on the Mbujimayi Market. International Journal of Health, Medicine and Nursing Practice, 8(3), 1–9. https://doi.org/10.47941/ijhmnp.3170

Issue

Section

Articles